首页> 外文OA文献 >Contrasting relations between diversity of candidate genes and variation of bud burst in natural and segregating populations of European oaks
【2h】

Contrasting relations between diversity of candidate genes and variation of bud burst in natural and segregating populations of European oaks

机译:欧洲橡树的自然种群和隔离种群中候选基因多样性与芽爆发变异之间的对比关系

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Nucleotide diversity was assessed within nine candidate genes (in total 4.6 kb) for the time of bud burst in nine sessile oak (Quercus petraea) populations distributed in central and northern Europe. The sampled populations were selected on the basis of their contrasting time of bud burst observed in common garden experiments (provenance tests). The candidate genes were selected according to their expression profiles during the transition from quiescent to developing buds and/or their functional role in model plants. The overall nucleotide diversity was large (pi(tot) - 6.15 x 10(-3); pi(silent) - 11.2 x 10(-3)), but population differentiation was not larger than for microsatellites. No outlier single-nucleotide polymorphism (SNP), departing from neutral expectation, was found among the total of 125 SNPs. These results contrasted markedly with the significant associations that were observed between the candidate genes and bud burst in segregating populations. Quantitative trait loci (QTLs) for bud burst were identified for 13 year*site seasonal observations in a cloned mapping pedigree. Nineteen QTLs were detected, and QTLs located on linkage groups 2, 5 and 9 contributed repeatedly to more than 12% of the phenotypic variation of the trait. Eight genes were polymorphic in the two parents of the pedigree and could be mapped on the existing genetic map. Five of them located within the confidence intervals of QTLs for bud burst. Interestingly, four of them located within the three QTLs exhibiting the largest contributions to bud burst.
机译:在分布于中欧和北欧的9个无柄栎(Quercus petraea)种群中,在9个候选基因(总计4.6 kb)的芽破裂时间中评估了核苷酸多样性。根据在普通花园实验(来源测试)中观察到的芽萌发对比时间来选择采样种群。根据候选基因从静止芽到发育芽的过渡过程中的表达概况和/或它们在模型植物中的功能作用来选择候选基因。总体核苷酸多样性很大(pi(tot)-6.15 x 10(-3); pi(silent)-11.2 x 10(-3)),但种群分化并不比微卫星大。在总共125个SNP中,未发现偏离中性预期的异常单核苷酸多态性(SNP)。这些结果与隔离群体中候选基因与芽爆发之间的显着关联形成了鲜明的对比。在克隆的地图谱系中,针对13年*站点的季节性观测结果确定了芽爆发的数量性状基因座(QTL)。检测到19个QTL,位于连锁组2、5和9上的QTL反复贡献了超过12%的性状表型变异。八个基因在谱系的两个亲本中是多态的,可以在现有的遗传图谱上进行定位。其中有五个位于芽破裂的QTL的置信区间内。有趣的是,其中三个位于三个QTL中,其中四个对芽爆发贡献最大。

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号